Bioremediation of Hexavalent Chromium Using an Indigenous Bacillus cereus: Molecular and Process Optimization Approach
DOI:
https://doi.org/10.31305/rrijm.2025.v10.n8.012Keywords:
Chromium, remediation, toxicity, waste water treatment, Bacillus cereusAbstract
Hexavalent chromium (CrVI) is highly toxic and carcinogenic heavy metal commonly found in industrial effluents, posing sever environmental and public health risks. The study explore the bioremediation of Bacillus cereus strain isolated from a chromium contaminated site. The strain demonstrated significant (CrVI) reduction capabilities under laboratory conditions. Molecular identification using 16S rRNA sequencing confirmed the identity of the isolate as Bacillus cereus. To enhance the bioremediation efficiency parameter such as pH, temperature, Chromium concentration, inoculum size and contact time were optimized using Response Surface Methodology. The optimization conditions resulted in over 70.97%. reduction within 24-96hours.Our findings suggested Bacillus cereus is a non-invasive, cost-effective, and environmentally friendly substitute for chromium treatment. It is also a significant implication for industrial uses and water treatment and may be a viable alternative to traditional remediation methods.
References
Thacher, R., Hsu, L., Ravindran, V., Nealson, K. H., & Pirbazari, M. (2015). Modeling the transport and bioreduction of hexavalent chromium in aquifers: Influence of natural organic matter. Chemical Engineering Science, 138, 552-565.
Karthik, C., Elangovan, N., Kumar, T. S., Govindharaju, S., Barathi, S., Oves, M., & Arulselvi, P. I. (2017). Characterization of multifarious plant growth promoting traits of rhizobacterial strain AR6 under Chromium (VI) stress. Microbiological research, 204, 65-71.
Ma, L., Xu, J., Chen, N., Li, M., & Feng, C. (2019). Microbial reduction fate of chromium (Cr) in aqueous solution by mixed bacterial consortium. Ecotoxicology and environmental safety, 170, 763-770.
Xu, W., Duan, G., Liu, Y. et al. Simultaneous removal of hexavalent chromium and o-dichlorobenzene by isolated Serratia marcescens ZD-9. Biodegradation 29, 605–616 (2018). https://doi.org/10.1007/s10532-018-9856-7.
Zhong, X., Sá, R. D. C. D. S. E., & Zhong, C. (2017). Mitochondrial biogenesis in response to chromium (VI) toxicity in human liver cells. International journal of molecular sciences, 18(9), 1877.
Yan, G., Gao, Y., Xue, K., Qi, Y., Fan, Y., Tian, X., & Liu, J. (2023). Toxicity mechanisms and remediation strategies for chromium exposure in the environment. Frontiers in Environmental Science, 11, 1131204.
Saud, S., Wang, D., Fahad, S., Javed, T., Jaremko, M., Abdelsalam, N. R., & Ghareeb, R. Y. (2022). The impact of chromium ion stress on plant growth, developmental physiology, and molecular regulation. Frontiers in Plant Science, 13, 994785.
Kholisa, B., Matsena, M., & Chirwa, E. M. N. (2021). Evaluation of Cr(VI) Reduction Using Indigenous Bacterial Consortium Isolated from a Municipal Wastewater Sludge: Batch and Kinetic Studies. Catalysts, 11(9), 1100. https://doi.org/10.3390/catal11091100.
Linlin Ma, Jinming Xu, Nan Chen, Miao Li, Chuanping Feng (2019). Microbial reduction fate of chromium (Cr) in aqueous solution by mixed bacterial consortium.Ecotoxicology and Environmental Safety,170 (15) 763-770. https://doi.org/10.1016/j.ecoenv.2018.12.041.
Yan G, Gao Y, Xue K, Qi Y, Fan Y, Tian X, Wang J, Zhao R, Zhang P, Liu Y and Liu J (2023), Toxicity mechanisms and remediation strategies for chromium exposure in the environment. Front. Environ. Sci. 11:1131204. doi: 10.3389/fenvs.2023.1131204
Sharma A, Kapoor D, Wang J, Shahzad B, Kumar V, Bali AS, Jasrotia S, Zheng B, Yuan H, Yan D(2020) Chromium Bioaccumulation and Its Impacts on Plants: An Overview. Plants (Basel). 13;9(1)100. doi: 10.3390/plants9010100. PMID: 31941115; PMCID: PMC7020214.
Linlin Ma, Jinming Xu, Nan Chen, Miao Li, Chuanping Feng (2019) Microbial reduction fate of chromium (Cr) in aqueous solution by mixed bacterial consortium. Ecotoxicology and Environmental Safety, 170, 763-770, 0147-6513. https://doi.org/10.1016/j.ecoenv.2018.12.041.
Sathishkumar, K., Murugan, K., Benelli, G (2017) Bioreduction of hexavalent chromium by Pseudomonas stutzeri L1 and Acinetobacter baumannii L2. Ann Microbiol 67, 91–98. https://doi.org/10.1007/s13213-016-1240-4.
Wang, G.-H., Cheng, C.-Y., Liu, M.-H., Chen, T.-Y., Hsieh, M.-C., & Chung, Y.-C. (2016). Utility of Ochrobactrum anthropi YC152 in a Microbial Fuel Cell as an Early Warning Device for Hexavalent Chromium Determination. Sensors, 16(8), 1272. https://doi.org/10.3390/s16081272
Yunfei Zhu, Junwei Yan, Li Xia, Xiang Zhang, Lixin Luo. (2019). Mechanisms of Cr (VI) reduction by Bacillus sp. CRB-1, a novel Cr(VI)-reducing bacterium isolated from tannery activated sludge. Ecotoxicology and Environmental Safety, 186, 109792, 0147-6513, https://doi.org/10.1016/j.ecoenv.2019.109792.
Karthik, C., Barathi, S., Pugazhendhi, A., Ramkumar, V. S., Thi, N. B. D., & Arulselvi, P. I. (2017). Evaluation of Cr (VI) reduction mechanism and removal by Cellulosimicrobium funkei strain AR8, a novel haloalkaliphilic bacterium. Journal of hazardous materials, 333, 42-53.
Tamura K, Stecher G, Kumar S(2021)MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol. 25; 38 (7):3022-3027. doi: 10.1093/molbev/msab120. PMID: 33892491; PMCID: PMC8233496.
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